Coding

Part:BBa_K2848003:Design

Designed by: Lars Hoeing, Liv Paul, Luca Schelle   Group: iGEM18_Stuttgart   (2018-10-09)


Pre-NisA containing internal "YAAY"- and His-Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was obtained from IDT and the final construct was obtained using Gibson Assembly and restriction cloning.


Source

Lactococcus lactis NZ9700

References